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Fig. 7. Functional downregulation of geminin results in significant increase in p53 and RAD51 level on chromatin. Embryos treated with δCdt1_193-447 were fixed and subjected to immunofluorescence with anti-p53 and anti-RAD51. Confocal/two-photon microscopy was performed with the same detection settings for both control and arrested cells. (A,C) Accumulation of p53 and RAD51 on chromatin compared with control by average fluorescent intensity or confocal/two-photon single cell imaging. Graphs represent mean±s.e.m. of two separate experiments with 6-8 embryos for each protein used for quantification, ***P<0.001. (B,D) Confocal/two-photon images comparing the amount of p53 (B) and RAD51 (D) between control cycling cells and cells arrested using δCdt1_193-447. Scale bars: 20 μm. |
