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tp53xenopus blastomere [+] 

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Experiment details for tp53

Wallingford JB et al. (1997) Assay

p53 activity is essential for normal development in Xenopus.

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Gene Clone Species Stages Anatomy
tp53.L laevis NF stage 29 and 30 blastomere

  Figure 3. Mutant p53 blocks differentiation. (a) Dorsal view of an embryo injected with p53Tthr280 mRNA plus beta-galactosidase mRNA into blastomere B1 of a 32-cell embryo, reared to tailbud stage, and stained with the anti-neural antibody XAN3/6F11 (dark blue/purple [48]). Anterior is to the left. Note that the beta- galactosidase-positive tumor (light blue) does not stain with the neural marker, and that the large tumor distorts the brain into which it is embedded. (b)Transverse section through a sample similar to that shown in (a). Neural staining (dark purple) with antibody 2G9 (XenopusMolecular Marker Resource, URL http://vize222.zo.utexas.edu) and beta- galactosidase (light blue). beta-galactosidase- positive cells do not stain with the neural marker, and vice versa. (c) Control side of a tailbud stage embryo stained (dark blue/purple) with the somite differentiation marker 12/101[49]. Note that the differentiated somites extend anteriorly to the head of the embryo. (d)The opposite side of the embryo shown in (c), illustrating a tumor derived from blastomere C2 injected with p53thr280mRNA plus beta-galactosidase mRNA. Note that the region in which anterior somites should have formed does not stain with 12/101, but is beta- galactosidase positive (light blue). (e)The control side of a tailbud stage embryo stained with antibody 3G8 [50] to detect pronephric tubules (dark purple substrate, arrow).(f)The opposite side of the embryo shown in (e), illustrating a tumor derived from the blastomere C3 injected with p53thr280mRNA plus beta- galactosidase mRNA. The region in which the embryonic kidney, the pronephros, should have formed does not stain with antibody 3G8. The beta-galactosidase-positive tumor (light blue) is displaced dorsally from the region in which the pronephros normally develops, but such displacement is common for mutant p53- induced tumors (see Figures 1 and 5).