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Fig. 3. SHP-2N308D leads to a delay of the cardiac cell cycle. (A-D) Transverse heart sections through stage 33 (A,B) and stage 37 (C,D) Xenopus embryos stained with Tmy (green) and anti-phospho histone H3 (red), from uninjected (A,C) and SHP-2N308D-derived (B,D) embryos (2 ng). Scale bar: 50 μm. (E,F) Graphs representing total number of Tmy-positive cells (E) and the mitotic index (F) in SHP-2FL- (blue bars) and SHP-2N308D- (red bars) derived embryos at stages 29, 33 and 37. Bars represent the average of at least three embryos. Error bars represent s.d. *P≤0.05 (comparison of SHP-2FL and SHP-2N308D embryos). |
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Fig. 5. SHP-2N308D leads to alterations in cardiac myofibril formation. (A-I) Transverse sections of stage 37 hearts from uninjected (A,D,G), SHP-2FL (B,E,H) or SHP-2N308D (C,F,I) Xenopus embryos (2 ng) immunostained for fibrillin (orange) and Tmy (green) (A-C), cardiac actin as detected by conjugated phalloidin (green; D-F) and tropomyosin (red) and DAPI (blue) (G-I). Scale bar: 100 μm. (J-M) Representative transmission electron micrographs of transverse images from stage 33 (J,K) or stage 37 (L,M) heart tissue derived from uninjected (J,L) or SHP-2N308D (L,M) embryos. Cardiac muscle fibrils are highlighted. Scale bars: 1 μm. |
