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tpm1xenopus   

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Experiment details for tpm1

Two skeletal alpha-tropomyosin transcripts with distinct 3''UTR have different temporal and spatial patterns of expression i...

Two skeletal alpha-tropomyosin transcripts with distinct 3'UTR have different temporal and spatial patterns of expression in the striated muscle lineages of Xenopus laevis.

Gene Clone Species Stages Anatomy
tpm1.L laevis NF stage 20 to NF stage 24 somite , muscle , epaxial muscle
tpm1.L laevis NF stage 26 to NF stage 28 somite , muscle , skeletal muscle , epaxial muscle
tpm1.L laevis NF stage 39 to NF stage 40 somite , heart , muscle , skeletal muscle , epaxial muscle

  Fig. 2. Developmental and spatial pattern of expression of XTMa2 and XTMa7 transcripts in embryo analysed by RNAse protection and wholemount in situ hybridization. (A) RNase protection analysis. Total RNA from different developmental stages embryos (9-42) or tRNA (2) was analysed by RNase protection with the XTMa27 probe that reveal simultaneously XTMa2 (339 nt protected fragment) and XTMa7 (214 nt protected fragments). (B) Whole-mount in situ hybridization was performed with XTMa2 and XTMa7 3'UTR specific probe and different developmental stages embryos as indicated.