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Fig. 2. Developmental and spatial pattern of expression of XTMa2 and XTMa7 transcripts in embryo analysed by RNAse protection and wholemount in situ hybridization. (A) RNase protection analysis. Total RNA from different developmental stages embryos (9-42) or tRNA (2) was analysed by RNase protection with the XTMa27 probe that reveal simultaneously XTMa2 (339 nt protected fragment) and XTMa7 (214 nt protected fragments). (B) Whole-mount in situ hybridization was performed with XTMa2 and XTMa7 3'UTR specific probe and different developmental stages embryos as indicated. |