| |
Figure 7. Inhibition of Myocardial Gene Expression by Expression of Dominant Negative Myocardin in Xenopus EmbryosXenopus embryos at the 8-cell stage were injected with mRNA encoding the Cδ585 mutant of myocardin and GFP or GFP alone (control) and were assayed for expression of (A) cardiac α-actin, (B) α-tropomyosin, or (C) Nkx2.5 at stage 28 by whole-mount in situ hybridization. (A, e), (B, c), and (C) show ventral views; other panels show lateral views. The dotted line bisects the symmetrical heart-forming region. Arrowheads show the heart on the injected side, which displays a reduced level of expression of cardiac markers. cg, cement gland; h, heart; s, somites |
