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FIGURE 11. GRAF1 depletion leads to fiber rupture. A, laser scanning confocal microscopy of mildly affected stage 34 somites with Tm (green) and laminin
(LAM) (red) reveal mid-somite tears (denoted by arrows). Laminin association with fiber tip is denoted by an asterisk. Scale bar, 100 m. B, TEM ( 2500
magnification) of somite-matched stage 37 Con Mo- and GRAF1 Mo-injected embryos. The myoseptum is denoted by a thick white arrow in the Con Mo panel.
The sarcolemma remained attached to the intersomitic boundary in GRAF1 morphants, but mid-myofiber tears were prevalent (black arrows). |
