| |
Fig. 3. A highly efficient method of testing promoters and creating transgenic lines using pTransgenesis. (A) Genomic PCR fragments are cloned directly into the p2 position, thus allowing the recombination shown. (B,C) PCR products encoding regions 5′ to nectin-2 and vimentin were generated and tested for transcriptional activity in F0 X. laevis and in F1 X. tropicalis. (D) Transverse sections through the hindbrain and spinal cord of embryos (dorsal side up) stained for endogenous vimentin expression (upper panels), or Venus transgene expression (green) in F0 transgenic X. laevis (middle panels) and F1 transgenic X. tropicalis (lower panels). (E) Transverse sections through the neural plate of stage 18 embryos (dorsal side up) stained for endogenous nectin-2 expression (upper panel), or Venus transgene expression (green) in F0 transgenic X. laevis (middle panel) and F1 transgenic X. tropicalis (lower panel). Nuclei are stained with DAPI (blue) in middle and lower panels in D and E. |
