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Fig. 5.
Double labeling for [3H]-thymidine and VIM immunohistochemistry. Tadpoles received [3H]-thymidine injections at S49 (A, B, D, and E) or 50 (C and F to J′). They were allowed to survive for 3 d (A to C and H to I′), 6 d (D), 9 d (E, J, and J′), or 30 d (F and G). The boxed areas in A, H, I, and J are further magnified in the next image. The arrowheads in C and D show the labeled fibers for VIM, which orientate to the magnocellular nucleus (Tmc). The brain levels of the figure located (cm: caudomedial, rm: rostromedial, and B1, B3, or B4) are shown in Fig. 1. Scale bar = 200 μm for J, 100 μm for A, H, and I and 50 μm for the others. For the abbreviations, see list. |
