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Fig. 6. The Nodal pathway does not regulate the Wnt/PCP pathway. (A,B) Xenopus embryos were injected with 10 ng Lefty recombinant protein, treated with 200 μM SU5402 or 200 μM SB-505124 at the indicated stages, and analysed by WISH at stage 12 (A) or 14 (B). SU5402, but not SB-505124, repressed Xbra and Xwnt11. All views are vegetal. (C) Four-cell embryos were marginally injected with a mixture of 250 pg/blastomere dsh-GFP and 1 ng/blastomere mcherry mRNAs in the two dorsal cells. Stage 12 embryos were fixed, sectioned, stained with an anti-GFP antibody and imaged with an apotome microscope. (D) The percentage of cells that displayed Dsh-GFP fluorescence predominantly at the cell membrane among the mCherry-positive cells. Three embryos were analysed per condition, and the total number of cells analysed is indicated. *, P=0.0314. |
