| |
Fig. 2. Inhibition of Xwnt11 expression by a dominant-interfering
Xbra construct. (A) Expression of Xwnt11 is induced in animal pole
regions by activin (8 units/ml) or Xbra-GR in the presence of DEX,
and induction is inhibited by Xbra-EnR (500 pg RNA).
(B,C) Downregulation of Xwnt11 by Xbra-EnR in the whole embryo.
Embryos were injected with 500 pg b-gal RNA (B) or 500 pg Xbra-
EnR RNA (C) into both blastomeres at the 2-cell stage. Expression of
Xwnt11 was examined by in situ hybridisation at the early gastrula
stage. Note that injection of Xbra-EnR RNA causes a dramatic
reduction in Xwnt11 expression, although expression in dorsal
superficial cells remains. |
