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Figure S2. Neither xANR5 mRNA nor xANR5-MO Inhibits Mesodermal Induction and Patterning (A) Ten DMZs overexpressing xANR5 were subjected to RT-PCR analysis. The overexpression of xANR5 did not alter the expressions of the mesodermal markers Xbra, Xwnt11, Xnot, and Xmyf5. The organizer marker chordin was also not changed by xANR5 mRNA microinjection. (B–D) WISH and RT-PCR were carried out to determine whether xANR5-MO injection affected mesodermal induction and patterning. No changes in gene expression, mesodermal induction, or tissue specification as a result of xANR5-MO microinjection were detected. A presumptive head mesodermal marker, Xgsc, and an axial mesodermal marker, XmyoD, were unaffected by xANR5-MO. RT-PCR analysis with mesodermal markers and FGF target genes also showed that xANR5-MO injection did not alter the gene expression patterns during early embryogenesis. (E) Mesodermal induction was tested in activin-injected animal caps. The expression of Xbra, which was induced by activin injection, was unaffected by the coinjection of ANR5-MO or hANR5. |
