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Fig. 1. A neural Wnt3a-Meis3 loop restricts hindbrain development. (A)Wnt3a expression in late-gastrula (a-c) or mid-neurula (d-f) embryos injected at the one- cell stage with either Meis3-MO (25 ng) or Meis3 mRNA (0.5 ng). In all figures, the phenotype frequency is indicated in each panel. (g)A schematic summary of the results. (B)Meis3 expression in late-gastrula (a-c) or mid-neurula (d-g) stage embryos injected at the one-cell stage with either THVGR (50 pg) or Dkk1 (35 pg) mRNAs. THVGR was activated by 1M dexamethasone (Dex) at gastrula (b,e,i) or mid-neurula (f,j) stages. Unactivated THVGR controls develop normally (insets in f, j). (k)A schematic summary of the results. See supplementary material Fig. S1. (C)EphA2 (a,b), n-Tub (d,c) and Slug (g,h) expression in mid-neurula stage DLMZ/NE recombinant explants (see text). For each recombinant: DLMZ is the pigmented-brownish tissue on the left/top (except in b, where it is on the back of the explant); NE is the albino tissue on the right/bottom. NEs are from embryos injected with BMP dominant-negative receptor (DNR) mRNA (160pg) and/or Wnt3a- MO (40ng). (c,f,i) The expression phenotype for pooled explants; for each group, 14-16 explants were examined per marker. |