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Fig. 5. hrt1 is induced by notch-ICD mRNA injection and promotes wnt4 expression. (A-E) X. laevis embryos were injected as previously described and left to develop to stage 28, when in situ hybridisation detecting expression of either wnt4 (A-C) or hrt1 (D,E) was performed. Embryos injected with hormone-inducible hrt1-hGR mRNA, but not incubated in dexamethasone to activate the message, have normal pronephric wnt4 expression (A). Embryos injected with hrt1-hGR mRNA but incubated in dexamethasone from stage 18 onwards, display ectopic wnt4 expression (B). MO depletion of HRT-1 inhibited wnt4 expression (C). Overexpression of notch-ICD causes a large increase in pronephric hrt1 expression (D), whereas hrt1 expression is largely unaffected by wnt4 mRNA injection (E). Asterisk denotes injected side. |
