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Figure 10. Zfp36 depletion does not affect somitogenesis nor early pronephros specification. (A) 20 ng of zfp36 morpholinos (MO) were injected into one dorsal blastomere of 4-cell stage embryos with 250 pg of lacZ mRNA. Embryos were fixed at stage 15 for the detection of myod by in situ hybridization (a) or at stage 28 for immunohistochemistry with 12/101 antibody (d). a, b and c are representative phenotypes and f and g are close up views of d and e respectively. Arrowheads indicate regularly segmented somites on the injected side. (B) 20 ng of zfp36 morpholinos (MO) were injected into one ventral blastomere of 8-cell stage embryos with 250 pg of lacZ mRNA. Embryos were fixed at stage 29/30 (a, b, e, f), 33/34 (c, d) or 27 (g, h) and analysed for the expression of lim1, pax8, wnt4 and wt1 by in situ hybridization. |
