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Fig. 7. Comparison of the
expression of Xwnt-4 and
Xwnt-1 in the neural tube of
a late tadpole stage embryo
(stage 28). (A) Antisense
Xwnt-4 and Xwnt-1 probes
hybridized to late tadpole
stage embryos and processed
for whole-mount in situ
hybridization. Embryos were
embedded in paraffin and
sectioned. Representative
sections are denoted by the
lettered arrows. (B) A
transverse section through an
Xwnt-4 hybridized embryo
taken at the level of the optic
vesicle (ov). Xwnt-4
expression occurs on the
dorsal midline of the
mesencephalon (me). (C) A
transverse section through an
Xwnt-4 hybridized embryo
taken at the level of the
auditory vesicle (av). At this
position in the
anteroposterior axis Xwnt-4
expression is seen in the
dorsolateral region of the
rhombencephalon (rh) and in
the ventral midline directly
above the notochord (nc).
The ventral portion of the
auditory vesicle is also
positive for Xwnt-4
expression. (D) A transverse
section through an Xwnt-4
hybridized embryo, taken at
the posterior edge of the
rhombencephalon. Xwnt-4
expression is localized to the
floor plate of the neural tube
(nt). (E) A transverse section
taken at the posterior edge of
the optic vesicle (ov),
through an embryo
hybridized with Xwnt-1.
Xwnt-1 expression occurs in
both the dorsal midline of
the mesencephalon (me) and
in the ventral-lateral margin
of the mesencephalon. (F) A
transverse section taken
through an Xwnt-1 hybridized
embryo in the
rhombencephalon (rh). All of
the Xwnt-1 expressing cells at
this point in the
rhombencephalon are
localized to the dorsal
midline. There is no Xwnt-1
expression in the ventral
midline overlying the
notochord (nc). |
