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Figure 4. Alexa 488–phalloidin fluorescence marks the F-actin cytoskeleton (green) of stereociliary bundles in confocal sections from S47 (A) and S50 (B) inner ear whole-mounts. Arrows point to stereociliary bundles. Propidium iodide counterstain (red) labels RNA (cytosol) and DNA (nuclei). A: S47 endorgan compartments contain regions where stereociliary bundles are identifiable. A1: Sacculus, utricle. A2: Crista of the anterior canal. A3: Lagena, cristae of posterior and horizontal canals. B: Representative confocal sections show that stereociliary bundles are visible in the epithelia of all S50 vestibular and auditory compartments. B1: Utricle, sacculus. B2: Cristae of horizontal and anterior canals. B3: Crista of horizontal canal (transverse view). B4: Lagena, sensory ganglion. B5: Basilar papilla, amphibian papilla, crista of posterior canal, sensory ganglion adjacent to amphibian papilla. Scale bar = 50 μm.Download figure to PowerPoint |
