| |
Fig. 2. RNAase protection analysis of XSTK9 expression. (A) Temporal
expression pattern. Total RNA from two embryos was extracted at the
indicated stages. (B) Spatial expression pattern at stage 8. RNA was
extracted from animal caps (An), marginal zone regions (Mz). and vegetal
poles (Vg). Yeast tRNA (tRNA) was used as a negative control for
protection. Pigment differences were used to define dorsal (DMz), lateral
(LMz) and ventral (VMz) marginal zones. To confirm these assignments,
additional explants were allowed to develop to stage 10, when they were
analyzed for expression of goosecoid. These results were observed in
three separate experiments. Samples were co-protected with EFlor to
control for amount of RNA loaded. The difference in appearance of
EFlor between the XSTK9 and goosecoid protections results from the
use of RNAase A with XSTK9. |
