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Fig. 3. Spatial expression patterns of aldolase A, B and C mRNAs examined in the wild-type Xenopus embryo at the tailbud stage (stage 35) by whole-mount in situ hybridization. Probes for aldolase A, B and C mRNA were, respectively, the HindIII fragment of XALDA (Hikasa et al., 1997), the AflII-XhoI fragment of XALDB2 (Kajita et al., 2000), and the SacI-KpnI fragment of XALDC (Atsuchi et al., 1994), which did not cross-hybridize to each other ( Kajita et al., 2000). All the signals were obtained with antisense probe, but not with the sense probe (data omitted). (a) An embryo hybridized with the XALDA antisense probe. Yellow lines indicate regions (b–d) where embryos were sectioned. h: heart anlage; p: pronephros, s: somites. In (b): b, brain; e, ear vasicle. In (c): s, somites; p, pronephros. In (d), somites are defferentially stained in dermatome (s (der)) and myotome (m (der)). (e) An embryo was fixed, cut into halves, and only the anterior half was hybridized with the XALDA antisense probe. Note the absence of difference in the staining between dermatome and myotome, indicating that the weaker staining in myotome in (d) is simply due to the lesser accessibility of the probe to it. (f) An embryo hybridized with the XALDB antisense probe. Yellow lines indicate regions (g–j), where embryos were sectioned. In (f–j): lr, liver rudiment; p, pronephros; ep, epidermis; pr, proctodeum. (k) An embryo hybridized with the XALDC antisense probe. Yellow lines indicate regions (l–n) where embryos were sectioned. In (k–n): br, brain; h, heart; p, pronephros; sc, spinal cord; rt, retina. |
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aldoa (aldolase A, fructose-bisphosphate) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 37 & 38, lateral view, anterior left, dorsal up (above), and in several transverse sections, as indicated (b,c,d, e: below). |
