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Fig. 1. Rspo3 is expressed in endothelial cells and their precursors. (A-K) Rspo3 expression by in situ hybridization during mouse and Xenopus embryogenesis. (A-D) Mouse embryo. (E-H) X. laevis. (I-K) X. tropicalis. (A) E8.0 embryo; anterior towards the left. Rspo3 expression is in the primitive streak and in the allantois. (B) E8.5 embryo; anterior towards the top. Rspo3 expression is prominent in the posterior mesoderm and in the allantois. (C) Rspo3 expression in vasculature of the chorionic plate (E8.5). (D) Expression in the umbilical vein (arrowhead) (E12.5). (E) Stage 17; dorsal view, anterior leftwards, showing expression in the neural plate. (F) Stage 28; lateral view with anterior leftwards, showing expression in organs labeled. (G,H) Transverse section at tailbud stage showing expression of Rspo3 (G) and Vegf (H) in the hypochord (arrowheads). (I-K) Expression of indicated genes in stage 24; ventral view, anterior leftwards, showing expression of Msr and Rspo3 in the periphery of the ventral blood islands. Abbreviations: all, allantois; ba, branchial arches; cp, chorionic plate; fb, forebrain; dnt, dorsal neural tube; dlp, dorsal lateral plate; lb, limb bud; n, notochord; pm, posterior mesoderm; pn, pronephric sinus; ps, primitive streak; tb, tailbud mesoderm; vbi, ventral blood island. |
