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Fig. 7. Arx represses Irx genes. All embryos are dorsal views at stage 14–15. Black and red arrowheads point at the control or injected side, respectively. (A) Double staining of Fezf2 (cyan) and Arx (purple). Both genes share their posterior limits (arrowheads). (B) Double staining of Irx1 (cyan) and Arx (purple) showing the complementary expression domains of these genes. (C) Injection of Fezf1 mRNA expanded Arx posteriorly. (D) In embryos injected with VP16-Znf-GR mRNA Arx expression is shifted anteriorly. (E–F) Overexpression of Arx (E) or Arx-EnR (F) mRNAs downregulated Irx1 (E) and Irx3 (F). (G) Impairment of Arx function with a specific MO expands Irx1 anteriorly. (H) Injection of Arx-VP16 mRNA caused a similar rostral expansion of Irx3. (I) Injection of VP16-Znf-GR mRNA expands anteriorly Irx3 expression. (J) This effect is reverted by overexpressing Arx. |
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Fig. 8. Irx repress Arx. All embryos are dorsal views at stage 14–15. Black and red arrowheads point at the control or injected side, respectively. (A) In embryos injected with Irx3-MT-GR mRNA Arx is downregulated in the presence of Dex. This effect was not observed in the absence of the hormone (not shown). (B) Impairment of Irx activity caused caudal expansion of Arx. (C, D) Injection of HG-GR-E1A mRNA caused posterior Arx expansion in the presence (C) but not in the absence of the hormone (D). (E–F) This expansion also occurred in the presence of cycloheximide. |
