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FIG. 4. The spatial distribution of pXen9 mRNAs prior to gastrulation. Methacrylate sections through embryos that had been hybridized
with a whole mount in situ schedule (see Materials and Methods). (A) 2 cell. (B) 4 cell. nuc, nuclei which have been lost during sectioning
because of their very large size. (C) 64-cell, animal pole (AP). (D) 64-cell, vegetal pole (VP). (E) Sense control, 2 cell. (F) Outermost animal
pole cells at stage 7. (G) Animal pole cells lining the blastocoel (blast) at stage 7 (early blastula). (H) Equatorial region of stage 9 (late
blastula) AN, animal pole; VEG, vegetal pole. blast, blastocoel. (I) Sense control: stage 7. Bars, 100 mm. |