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Fig. 3. Atp2a2/serca2 and atpa3/serca3 embryonic expression. (A). Wholemount in situ hybridization (a–j, j′, o–r, r′) or transverse sections (g′–n and o′–v) are shown for atp2a2/serca2 (a–c, g–n) and atpa3/serca3 (d–f, o–v). Neither genes are significantly expressed at cleavage and gastrulation stages (a–f). Neurula (g–h′; o–p′) show serca2 expression in the neural tissue (arrow1) but not in the neural crest (arrow 2); while serca3 is found in the ectoderm. Tadpoles (stage 26, i–i′ and q–q′; stage 37, j–n and r–v) show dynamic serca2 expression in the neural tube (arrow 1, section in i′, i′, l), the cement gland (arrow 3), the eye (section in k), the pronephros (arrow 4, section in i′, n), the heart and branchial arches mesenchyme (arrow 6, section in m) and the proctodeum (arrow 5). Serca3 is restricted to the ectoderm (q–q′), the cement gland (arrow3, q, r, s) then to the sensory ectodermal cells (r–v). See text for details. Scale bar: a–j, j′, o–r, r′ 0.5 mm; g′–n, o′–v 0.1 mm. (B). Whole mount in situ hybridization (b,c,d) on embryos at stage 37–38 for atp2a2/serca2, nkcc2 and evi1 and scheme of the structure of the pronephros at tadpole stage (a, modified from (Raciti et al., 2008)). Atp2a2/serca2 is expressed in the proximal part of the tubule (PT); nkcc2 is expressed in the distal part of the tubule (DT); evi1 is expressed in the distal part of the tubule and in the duct (D). Scale bar: b–d 0.5 mm. |
