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Figure 1. ATP4a Is Symmetrically Expressed and Required Post-MBT for LR Development(A�E) Whole-mount in situ hybridization of staged embryos at the (A) two-cell, (B) four-cell, and (C) 32-cell stages. Note that ATP4a mRNA was enriched in the animal cytoplasm, as shown in the bisected four-cell embryo in (B), viewed from the inner surface in (B′) and (B�), but symmetrically expressed with respect to the LR axis. (D) Zygotic ATP4a expression in the stomach of a stage (st.) 45 tadpole; see plane of section in (D′) indicated by dashed line in (D). Arrowhead indicates gastric epithelium in blow-up. (E) Four-cell embryo stained with sense control RNA.(F and G) (F) Pitx2c expression and (G) organ situs in wild-type embryos at tadpole stages (26�32) and in specimens injected or treated as specified. The image in (D′) was reconstructed from several images taken from the same histological section. ∗∗Highly significant. ∗∗∗Very highly significant. ns, not significant; n, number of embryos analyzed; wt, wild-type.See also Figure S1.
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atp4a ( ATPase, H+/K+ exchanging, alpha polypeptide ) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 2, animal view. |
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atp4a ( ATPase, H+/K+ exchanging, alpha polypeptide ) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 3, animal view. |
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atp4a ( ATPase, H+/K+ exchanging, alpha polypeptide ) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 6, animal view. |
