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Fig. 5. Spatial expression pattern of complement component C3 (C3). C3 signals
were not detected by late gastrula stages (A). Its expression appeared at the anterior
border of the neural plate, and then became restricted at the NC (B, C). NC expression
was confirmed by sectioning (D). C3 expression in the NC declined quickly, and
an expression domain gradually appeared at the ventral region of the trunk (E,F).
Sections from stage 32 embryos indicated that C3 was expressed in the somatic and
splanchnic layer of lateral plate mesoderm just internal to the epidermis. (G), sagittal
section; (H), transversal section. Endodermal expression is prominent at later stages
(I). Arrow points to an expression domain in the heart primordium. Ac, archenteron; bl,
blood island; edy, endodermal yolk mass; ep, epidermis; lpm, lateral plate mesoderm;
nc, neural crest; nt, neural tube; nd, notochord; sm, somite; vm, ventral mesoderm. |