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Fig. 3. CARTp-immunoreactive perikarya in the diencephalon and
the mesencephalon. A: Immunoreactivity in the rostral diencephalon.
Note stained cells in the nucleus of Bellonci (B; arrows). B: Immunoreactive
cells and fibers in the infundibulum (inf) and the dorsal
hypothalamic nucleus (dHy). C: Stained cells of the magnocellular
preoptic nucleus (Mg) in the wall of the infundibulum (inf). Note that
their long dendrites are oriented laterally, toward the surface of the
brain. D: Immunoreactive cells in the hypothalamus and the Edinger-
Westphal (EW) nucleus and intensely stained axon terminals in the
neural lobe of the pituitary gland (pn). Sagittal section. Rostral is to
the left. E: Immunostained infundibular neurons with long dendrites
at the level of plane E in Figure 1. Note stained perikarya in the
ventromedial (VM) and the posterior entopeduncular nuclei (epp).
F: Immunostained fibers in the pars nervosa (pn) of the pituitary
gland and median eminence of the hypothalamus. The intermediate
pituitary lobe (pi) is immunonegative, whereas some cells in the distal
lobe (pd) reveal weak immunoreactivity. G: Detail of the median
eminence, with small and less numerous stained dots in the internal
zone (iz) and large stained dots and short varicose fibers in the
external zone (ez). Hd, dorsal habenula; Hv, ventral habenula. 3rd,
third ventricle. Scale bar 200 m in A,B; 100 m in C,E; 300 m in
D; 150 m in F; 15 m in G. |
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Fig. 6. Immunoelectron microscopy of CARTp immunoreactivity
in the neural lobe of the pituitary gland of X. laevis. A: Conventional
fixation. Low-power micrograph with neurohemal axon terminals (a)
and a capillary (c) with endothelial cell (e) and blood cell (b). B: Conventional
fixation. Round secretory granules (g) in neurohemal axon
terminal, with some immunogold particles (arrowheads) indicating
the presence of CARTp. C: High-pressure freezing and cryosubstitution
followed by silver intensification of postembedding immunogold
labeling, showing strongly immunopositive neurohemal terminals (a).
D: As in C but the axon terminal is shown in detail. E: As in C but
immunonegative, as sections were processed with antiserum preadsorbed
to CARTp. s, intercellular space. Scale bar 1 m in A,C; 100
nm in B; 250 nm in D; 300 nm in E. |
