| Search Criteria |
| Gene/Clone | Species | Stage | Anatomy Item | Experimenter |
|---|---|---|---|---|
| cdh3 | xenopus | blastomere [+] |
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Experiment details for cdh3
Of mice, frogs and flies: generation of membrane asymmetries in early development.
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| Fig. 2. Membrane asymmetry in early Xenopus laevis blastomeres is independent of Ca2+-dependent cell adhesion. (A) A 128-cell X. laevis embryo raised in Ca2+- free medium. The cells form a loosely attached aggregate. (B) Single blastomeres from 64-cell stage X. laevis embryos. Note the dark pigment at the apical domain of the polar blastomeres. (C) Single blastomeres from an embryo that was surface biotinylated before cleavage and then raised in Ca2+-free medium (for details see Müller & Hausen 1995). The cells were fixed and stained with antibiotin antibodies (orange). Note extensive label associated with the apical membrane domain. The large arrow points to the border of the apical and the lateral membrane domain. The small arrows mark yolk platelets, which exhibit non-specific autofluorescence. Arrowheads indicate autofluorescence produced by the pigment granules, which are concentrated in the apical cytocortex. (D) Immunolabeling of XB/U-cadherin (green) is restricted to the basolateral domain of isolated blastomeres. XB/U-cadherin is specifically localized on the basolateral membrane domain. The arrow points to the apicallateral border of the membrane domains. In both panels (C) and (D), the apical domain is up and the basal domain is facing the bottom of the panel. (E,F) Transmission-electron micrographs of the apical-lateral border of isolated blastomeres. The apical domain is marked by the dark pigment granules (pg, arrows in E) and the presence of numerous microvilli (mv). The boxed area in (E) is enlarged in (F). The apical-lateral border is composed of a dense cortical cytoplasm, enriched in vesicles (arrowheads). The plasma membrane is continuous with a membrane invagination (arrows), which forms at the border of apical and lateral membranes. |