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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
cirbp
|
|
laevis
|
NF stage 10
|
ectoderm
,
animal cap
|
cirbp
|
|
laevis
|
NF stage 16
|
pre-chordal neural plate
,
chordal neural plate
,
intermediate mesoderm
,
neural plate
,
anterior neural fold
|
cirbp
|
|
laevis
|
NF stage 22
|
eye
,
posterior neural tube
,
pronephric mesenchyme
,
anterior neural tube
,
cranial neural crest
,
[+]
|
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Additional File 2. Expression of XCIRP during early embryogenesis. (A) Spatial expression of XCIRP during early development as revealed by in situ hybridization. The arrows indicate high expression of XCIRP in neural tissue. Arrowheads point to the expression in the branchial arches. The red arrow indicates staining of the pronephros. The open reading frame of XCIRP was amplified using the following primers: XCIRPstart: 5'-tcagaattcaatgtctgacgaaggaaaact-3' and XCIRPstop 5'-cttctcgagttactcgtgtgtagcatagctg-3' and sub-cloned into pGEMT for creating labeled antisense RNA. (B) Temporal expression of XCIRP and XCIRP2 as revealed by RT-PCR. H4 indicates the amplification of the house keeping gene histone 4, -RT the amplification of H4 in samples, which have not been reverse transcribed. The following primer pairs were used: histone 4: 5'-cgggataacattcagggtatcact-3' and 5'-atccatggcggtaa ctgtcttctt-3'; XCIRP: 5'-gctgatcaggcggggccacc-3' and 5'-gcacccaggctctgtcctgc-3'; XCIRP2: 5'-ccattcaggctgatcagg-3' and 5'-ctggagagagacgaacac-3'. |