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Fig. 1.
Temporal and spatial expression pattern of Xclaudin 1 during Xenopus development. (A) Temporal expression pattern of Xclaudin 1 was detected by RT-PCR. Ornithine decarboxylase (ODC) primers were used to normalize the amount of cDNA template. (B) RT-PCR of gastrula stage embryos (st.10.5). Xclaudin 1 mRNA is enriched in animal pole region and dorsal marginal zone in embryos. Chd (chordin) and BMP 4 were used as each gene control of explanted tissue and EF1α also used as a loading control. (C–J) In situ hybridization of spatial expression of Xclaudin 1 during early Xenopus development. Whole-mount in situ hybridization was carried out using a digoxigenin-labeled riboprobe. (C) Oocyte, animal view. (D) 2-cell stage, animal view and vegetal view. (E) 4-cell stage, animal view and vegetal view. (F) One hundred and twenty-eight cell stage, animal view and vegetal view. (G) Gastrular stage, animal view. (H) Neurula stage, animal view. (I) stage 29/30 lateral view. pd, pronephric duct; pn, pronephrus; ov, optic vesicle; df, dorsal fin; ev, eye vesicle; br, branchial arch. |
