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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
col1a2
|
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tropicalis
|
NF stage 54
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vertebra
,
perichondrium
|
col2a1
|
|
tropicalis
|
NF stage 54
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notochord
,
chondrocyte
,
vertebra
,
perichondrium
,
vacuolated notochordal tissue
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col2a1
|
|
tropicalis
|
NF stage 57
|
notochord
,
chondrocyte
,
vertebra
,
vacuolated notochordal tissue
|
col1a1
|
|
tropicalis
|
NF stage 54
|
vertebra
,
perichondrium
|
col1a1
|
|
tropicalis
|
NF stage 57
|
vertebra
,
periosteum (sensu osteogenic layer)
,
osteoblast
|
|
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Figure 5. Skeletal expression patterns the Xt-Col1a1, Xt-Col1a2, and Xt-Col2a1 during Xenopus tropicalis vertebrae development. Transverse sections of stage NF54 (A–F) and NF57 (G–L) vertebrae processed for in situ hybridizations using the Xt-Col1a1, Xt-Col1a2, and Xt-Col2a1 probes, as indicated. Black arrowheads show loose (A,B) or perichondral (D–F) cells. White arrowheads in (C,I) show Xt-Col2a1 positive epithelial non-vacuolated cells of the notochord. Arrows point at osteoblasts expressing Xt-Col1a1 (G,J), Xt-Col1a2 (H,K), or Xt-Col2a1 (I,L). In (I,L), calcified, Alizarin red-positive cartilaginous regions are marked by an asterisk and the dotted lines demarcates expression boundaries between Xt-Col2a1 positive and Xt-Col2a1negative chondrocytes. In situ hybridization signal is light to dark blue. Brown endogenous X.t. pigment cells are also visible in the vicinity of the dorsal neural arch (D–F, J–L). Scale bar in (A) represents 50 μm in (A–F); scale bar in (G) represents 50 μm in (G–L). |
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Figure 3. Comparison of the Col1a1, Col1a2, and Col2a1 expression patterns during Xenopus tropicalis hindlimb development. Stage NF54 (top panel) or NF60 (bottom panel) hindlimbs were examined by whole mount Alizarin red staining (insets), sectioned along the proximo-distal axis and stained with HES, (A–C, M–O) or processed by in situ hybridization for the Xt-Col1a1, Xt-Col1a2, and Xt-Col2a1 probes, (D–L, P–X). Results are shown for the whole skeletal element (left column, scale bar: 500 μm) and higher magnifications of the diaphysis (middle column, scale bar: 50 μm) and epiphysis (right column, scale bar: 50 μm). Arrows and arrowheads show osteoblasts and osteocytes, respectively. In situ hybridization signal is light to dark blue, and brown endogenous X.t. pigment cells are visible on most sections. Legend: Bo, bone; Ch, chondrocytes; Me, medulla; Pe, perichondrium; Sm, striated muscles. |