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Fig. 6
Effect of Ly-294,002 injection into mandibular arch precursors on cartilaginous cranial morphology in X. laevis larvae. 3D-reconstructions based on confocal laser scanning microscopy of a control and b injected specimens at ZO 17 in lateral view. Maximum intensity projections of c control and d injected specimens at ZO 20 stained with monoclonal II6B3-collagen II antibody and Alexa 568 in dorsal view. The reduction of the processus muscularis and the presence of an ectopic cartilage can be observed in injected specimens. ch, ceratohyal; cm, Meckel’s cartilage; ec, ectopic cartilage; pmpq, processus muscularis of the palatoquadrate; pq, palatoquadrate. Scale bar 200 μm |