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Fig. 1. Temporal and spatial expression of dazap2 mRNA. (A) RT-PCR using dazap2 primers shows that dazap2 is expressed during blastula to neurula stages. (B-I) Dazap2 spatial expression pattern was analyzed by whole-mount ISH, using either antisense (B, C, E-G and H, I) or sense (D and G′) full-length xl-dazap2 probe. Embryos are seen in dorsal view with anterior to top (B and D, E), posterior view with dorsal on top (C), lateral view with head to the left (F, G, G′ and H) or ventral view (I). The blue arrow indicates the blastopore. Embryos hybridized with the sense probe in the same ISH conditions show no staining at all stages tested (D and G′). (B) At mid-neurula stage 16 (B) and late neural stage 18 (C, posterior view), dazap2 is present in the head folds (red arrowhead) and throughout the neural plate (black arrow). The staining is enhanced when neurulae are cleared (stage 19, E and F). By stage 32 (G, G′), tailbud embryos express dazap2 in trunk and head region, including the brain (red arrowhead) and spinal cord, the eye, the branchial arches (arrowhead) and the pronephros (green arrow). A faint staining is found in the dorsal fin (arrow) and the proctodeum (blue arrow). Cleared stage 44 tadpoles (H, I) express dazap2 in the brain (red arrowhead), the pronephros (green arrows) and caecum (blue arrow). |
