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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
ddx39a
|
|
laevis
|
NF stage 22
|
eye primordium
,
neural plate
,
cranial neural crest
,
neural tube
|
ddx39a
|
|
laevis
|
NF stage 26
|
otic vesicle
,
mandibular crest
,
hyoid crest
,
branchial crest
,
neuroectoderm
,
[+]
|
ddx39a
|
|
laevis
|
NF stage 28
|
mesoderm
,
tail region
,
pharyngeal arch
,
branchial arch
,
optic vesicle
,
[+]
|
ddx39a
|
|
laevis
|
NF stage 37 and 38
|
otic vesicle
,
lens
,
spinal cord
,
neuroectoderm
,
mesoderm
,
[+]
|
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Fig. 3. Ddx39 expression was detected in the developing neural plate, neural tube, branchial arches, and otic vesicls. WISH was performed on embryos from st 17 to 28. Photomicrographs of wholemounts and sections through the wholemounts for st 17 (A and B), 22 (C), 26 (D and E), and 28 (F and G) are shown. Ddx39 expression was restricted primarily to the developing nervous system by stage 17 (A). This pattern was confirmed by section analysis (B) which showed labeled neural plate (NP) and no expression in the ectoderm (Ec) and endoderm (En). The head mesoderm (Ms) appeared to be very lightly labeled. The neural tube, including the optic cups (OC), the branchial arches and otic vesicle were labeled at st 22 (C) and 26 (D). WISH using sense control probe showed only weak background levels of labeling (E). Stage 28 tadpoles showed similar labeling patterns to those obtained at earlier stages including expression in the facial mesenchyme and pharyngeal arches and higher levels of expression in the otic vesicle, optic cup, and neural tube proper (F and G). WISH and section analysis at st 38 showed Ddx39 to be expressed in the neural tube (NT), otic vesicles (OV) and head mesenchyme (Ms), and was absent in notochord (Nc), endoderm (En), and heart (HT, H,I). Abbreviations are as follows: NP; neural plate, Ec; ectoderm, En, endoderm, Ms; mesoderm, OV; otic vesicle, BA; branchial arches, Ph; pharynx. Magnification Bar in (B) =50 μM and is applicable to photomicrographs in (B), (G) and (I). |