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eif5b (eukaryotic translation initiation factor 5B) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 20, anterior view, dorsal up. |
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Supporting Figure S1: Expression pattern of BG161338 (eif5b) , which was up-regulated in the microarray assay, but not validated by RT-PCR or ISH assays. A: RT-PCR analysis of unmanipulated embryos shows that BG161338 is highly expressed at all developmental stages investigated. B: ISH analysis shows that at gastrula stages, BG161338 is expressed in the animal cap ectoderm (an) and the marginal zone (bracket) that will give rise to mesoderm; side view with animal to the top. Veg, vegetal pole. At neural plate stages, it is strongly expressed in the neural plate (np) and the ectoderm bordering the neural plate (arrow); anterior view with dorsal to the top. At neural tube stages, it is strongly expressed throughout the neural tube (nt), in the migrating neural crest (nc), and in the lens (L); anterior view with dorsal to the top. At larval stages, it is additionally expressed in the otocyst (oto), branchial arch mesoderm (BA), somites (so), nephric mesoderm (ne), and ventral blood islands (bl); side view with anterior to the left. |
