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Fig. 4. Anos1 is essential for the formation of cranial placodes and their derivatives. (A–D) Unilateral injection of anos1MO (40 ng) at the 2-cell stage causes a reduction/loss of expression of two pan-placodal genes, six1 (A) and foxi4.1 (B), as well as genes restricted to individual placodal domains such as dmrta1 (C) and pax8 (D). (E–G) Later in development, the injected embryos exhibited reduce dmrta1 (E), emx2 (F) and ebf2 (G) expression in the olfactory epithelium. (H–I) The expression of foxe1 in the developing pituitary was also reduced (I) as compared to sibling control (H). (A–I) Anterior views, dorsal to top. The injected side (arrowheads) is to the right. The percentage of affected embryos is indicated in the upper right corner of each panel. |
