|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
hoxb3
|
|
laevis
|
NF stage 21
|
hindbrain
,
neuroectoderm
,
rhombomere R5
,
rhombomere R6
|
meis3
|
|
laevis
|
NF stage 21
|
hindbrain
,
spinal cord
,
neuroectoderm
,
anterior
|
egr2
|
|
laevis
|
NF stage 21
|
hindbrain
,
neuroectoderm
,
cranial neural crest
,
rhombomere R3
,
rhombomere R5
|
hoxb9
|
|
laevis
|
NF stage 21
|
spinal cord
,
neuroectoderm
,
central nervous system
|
epha2
|
|
laevis
|
NF stage 21
|
hindbrain
,
neuroectoderm
,
tail region
,
rhombomere R4
|
|
|
Fig. 6. XMeis3 antisense morpholino oligonucleotide eliminates hindbrain marker expression. Two-cell albino embryos were injected unilaterally into the animal hemisphere of one blastomere with 6-7.5 ng of the XMeis3 AMO. In situ hybridization was performed in late neurula stage embryos. In all cases, embryos are viewed dorsally; embryos are oriented anterior (top) to posterior (bottom). The red arrow delineates the dorsal midline. Embryos were injected on the right side. (A) In situ hybridization with Krox20 and HoxB9; Krox20 expression is eliminated on the AMO-injected side. HoxB9 expression is unchanged on the AM- injected side. (B) In situ hybridization with XE10; expression is eliminated on the AMO-injected side. (C) In situ hybridization with HoxB3 and HoxB9; HoxB3 expression is eliminated on the AMO-injected side. HoxB9 expression is unchanged on the AMO-injected side. (D) In situ hybridization with XMeis3 and En2 (red); expression of both markers is posteriorized on the AMO-injected side. (E) In situ hybridization with XMeis3; expression is inhibited on the AMO-injected side. The XMeis3 expression in r2 is indicated by arrows on both sides. |