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Figure 1. Xeya3 is expressed in the anterior neural plate during Xenopus development. Whole-mount in situ hybridization of Xenopus oocytes and embryos. A: Stages I-V of oogenesis. Xeya3 transcripts become localized to the animal hemisphere in stage V oocytes. B: Lateral view. In blastula stages, Xeya3 transcripts are enriched in the animal pole. C: Anterior view. During neurulation, Xeya3 expression in the anterior neural plate includes the eye field (black arrowhead). D: Anterior view. At the end of neurulation, strong Xeya3 expression is found in the eye vesicles (black arrowhead) as well as cells of the lens placodes (red arrowhead). E-G: Lateral view, anterior to the right and (E prime ) horizontal (F,G prime ) transversal sections. E,G: In tadpole stage embryos, cranial neural crest cells (black arrowheads in E,E prime ) migrate toward the branchial arches (black arrowheads in G). Xeya3 is expressed in the eye vesicles/cups in early but not late tadpole embryos (white arrowheads in E-G) and in the otic vesicle (white star in E). Within the mid- and hindbrain, Xeya3 transcripts are localized dorsally (black arrowhead in F). G-G prime : Strong Xeya3 expression resides in primary lens fibers (red arrowhead in G) and in the branchial arches (black arrowheads in G). ap, animal pole; ba, branchial arches; fbv, forebrain ventricle; le, lens; eyf, eye field; eyv, eye vesicle; lp, lens placode; mnc, migrating neural crest; nr, neural retina; plf, primary lens fibers; st, embryonic stage; vp, vegetal pole. Staging according to Nieuwkoop & Faber ([1967]). |
