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FIGURE 6. Analysis of progenitor cells at embryonic stages (BLBP, Lhx2, Sox2, Pax6, PH3). Micrographs of transverse sections through the pallium of Xenopus laevis at embryonic stages showing the codistribution of the progenitor markers BLBP (A,C), Lhx2 (B,C,E–G), Sox2 (D), and Pax6 (E,F′); and the combination of Lhx2 with the mitotic marker PH3 (G,H). In each panel the developmental stage and the color code for the used markers are indicated. (F,F′) Are confocal images, and the higher magnification shown in (F’) corresponds to the framed area indicated in (F). The BLBP (A) and the Lhx2 (B) labeling detected at stage 37/38 showed double labeled cells exclusively in the ventricle (C). The combination of Lhx2 and Sox2 also showed double labeled cells in the ventricle, but the cells detected for both markers in the mantle were never double labeled (D). In the case of Pax6, Lhx2/Pax6 double labeled cells were observed in the ventricular zone (E,F′), but never away from this region, where both cell populations were intermingled (F′). The labeling of Lhx2 in combination with PH3 showed that only the ventricular cells expressing Lhx2 were mitotic cells (G,H). Scale bars = 50 μm. See list for abbreviations. |
