Search Criteria |
Gene/Clone | Species | Stage | Anatomy Item | Experimenter |
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fbn1 | xenopus | endocardium [+] |
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Experiment details for fbn1
Spatiotemporally Controlled Mechanical Cues Drive Progenitor Mesenchymal-to-Epithelial Transition Enabling Proper Heart Form...
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Figure S7. Microsurgical manipulations to visualize HPCs in Xenopus. Related to Experimental Procedures. (A) Schematic of microsurgical manipulations to visualize HPC migration. (B) Microsurgical isolates at 1 h and 24 h. (C) Ventral windowed embryo at 1 h and 24 h. (D) Lateral windowed embryo at 1 h and 24 h. Yellow arrows mark location of beating heart. Scale bar = 500 μm. (E) Stage 33 embryo and equivalent stage microsurgical isolate stained for troponin T (red), fibrillin (white) and nuclei (green). Scale bar = 50 μm. | |||||||||||
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Figure 2. (A–C) Modulators of tissue compliance applied during stages of early heart development exhibit defects, including pericardial and neural edemas (A) (see arrows; scale bar, 1 mm), altered AP length (B), and increased rates of edema per clutch (C) (n = 30–35 embryos over four clutches). (D) Compliance measured by microaspiration of HFR. (E) Compliance at stage 22 confirms that blebbistatin and Y27632 increase and calyculin A decreases compliance (n = 11–17 embryos per treatment over three clutches). (F) Transverse sections through HFR at stage 28 show changes in polarity (aPKC or ZO-1) within the progenitor population (red). Lower panels show the epithelial marker masked using tropomyosin expression (scale bar, 50 μm). (G) Apical intensity after small-molecule inhibitor treatment (n = 9–13 embryos over four clutches). (H) Representative lateral confocal sections of stage 39 tadpole hearts (scale bar, 100 μm). (I) Cardiac anatomy after stage-specific inhibitor treatments as shown in Figure S2 (n = 5 embryos per treatment per period). Error bars represent mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. See also Figures S1, S2, and S3A. |