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Figure 4. Localization of XF protein in the embryo. XF protein is detected by immunostaining. Anterior is to the left in A-D and G. Dorsal is up in D-F, and G is a dorsal view from above. A: Dorsal view of St-11 whole mount. White squares identify the location of the blastopore lip. B: Dorsal view of St-12 whole mount. C: Dorsal, mid axial-posterior close-up of St-13 whole mount. D: Lateral view of a St-22 whole mount. E: Cross-section through mid-axis of St-22 embryo. Scale bar is for E and F. F: Adjacent cross-section through mid-axis of St-22 embryo, revealing periodic, orthogonoal intersomitic staining. G: Dorsal view of a St-39 embryo. H: Western blot of St-16 embryo extract probed with the JB3 antibody revealing a single band in the size range of fibrillin isoforms (350 kD), as well as a second, smaller band (200 kD). YP, yolk plug; N, notochord; SM, somitic mesoderm; NT, neural tube; IS, intersomitic space; E, eye; B, brain. Scale bars = 0.125 mm (C, E/F), 0.25 mm (A,B), and 0.5 mm (D,G). |
