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Fig. 5. Blastema cell proliferation and effects of growth factor (A) Bmp7, fgf2, fgf8, and shh expression in Xenopus DRG were visualized by in situ hybridization. (B) Phosphorylated Smad1/5/8 (pSMAD) and phosphorylated ERK (pERK) were detected by western blot analysis. α-tubulin is an internal control. (C) A quantitative PCR analysis of proliferating cell nuclear antigen (pcna). Statistical significance was determined via ANOVA followed by Bonferroni's multiple comparison test. (**), significantly different from each group. Bars represent standard deviation of three technically repeat experiments. |
