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Fig. 6. The preorganizer graph. (A) The data for the maternal h-Catenin and the Nodal-related signaling pathways at blastula stage (8.5 h post fertilization) were plotted as in Fig. 4. Color coding indicates the spots encoding chordin, Xnr-3, pintallavis/FoxA4a, and several novel genes. Since the spot intensities using blastula stage RNAs were weaker than those at gastrula stage, background correction distorted the clone distribution and therefore this step was omitted. (B E) Whole-mount in situ hybridizations with pintallavis/FoxA4a on untreated (B, D) and LiCl-treated (C, E) blastula stage embryos, (B, C) animal (D, E) and lateral views. Like other preorganizer genes, pintallavis/FoxA4a expression is expanded over the entire animal cap upon activation of the maternal h-Catenin pathway. |
