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Figure 3: WDR5 has chromatin dependent and independent roles in ciliogenesis in the
LRO
(a) In situ hybridization for foxj1 in the Xenopus LRO in uninjected controls and wdr5
morphants at stage 16. Embryos were bisected to reveal expression of foxj1 in the entire
GRP (left panels, dorsal to top and ventral view so right margin of LRO is to the left. Margin
of GRPs marked by white lines). GRPs were again bisected within the LRO (dashed white
line) to reveal foxj1 expression within the tissue (right panels, dorsal to the top and arrows
mark the ventral surface indicated by dashed white box at the bottom where the LRO resides
and showing loss of foxj1 in the ventral tissue). D, dorsal, V, ventral.
(b) Percentage of embryos that have abnormal foxj1 expression in control and wdr5
morphant Xenopus LROs. |
