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Fig. 2. Spatial expression patterns of XFrs3. (A) Whole-mount in situ hybridization of XFrs3. Embryos at indicated stages (13, 15, 20, 25, 28 and 35) were subjected to in situ hybridization with XFrs3 antisense probe. For stages 25, 28 and 35, right panels show magnified images of embryos at left panels. At early neurula stage (stage 13), XFrs3 is expressed broadly in the presumptive anterior neuroectoderm, and its expression is gradually localized to the eye field, cement gland and neural tube at mid-neurula and late neurula stages (stages 15 and 20). At early tailbud stages (stages 25 and 28) and tadpole stage (stage 35), XFrs3 transcript is localized in anterior structures of neuroectodermal origin [branchial arches (ba), cement gland (cg), eyes (e) and otic vesicles (ov)], brain (b), spinal cord (sc), somites (s) and pronephros (pn). (B) XFrs3 expression pattern in the eye at stage 35. Embryos at stages 35 were subjected to whole-mount in situ hybridization with XFrs3 antisense probe and then cross-sectioned. XFrs3 is expressed in the lens, the retina, and the brain. |
