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Figure 1. Analysis of temporal and spatial patterns of expression of xFurin in developing Xenopus embryos. A: Schematic illustration of domain structure and amino acid identity shared by Xenopus and mouse furin. B: Sequence alignment of the cytoplasmic tail domain of Xenopus and mouse furin showing conservation of internalization signals (green shading), acidic clusters (yellow shading), and phosphorylated serines (black dots) that direct subcellular trafficking. C-F: Analysis of xFurin expression by Northern blot analysis (C) or whole-mount in situ hybridization (D-F). D: Dorsal (dor), lateral (lat), anterior (ant), and midsagittal (lower right) views of neurula stage (st. 18) embryos are shown. Sm, somitic mesoderm; lp, lateral plate mesoderm; vm, ventral mesoderm. E,F: Lateral views of tail bud stage (st. 28-32) embryos. The embryo shown in F has been cleared. pn, pronephric duct; ov, otic vesicle; m, fin mesenchyme; pa, pharyngeal arches; e, eye. |
