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Fig. 3.
Relationship between eif6 and gipc2 expression. a. In situ hybridization performed using gipc2 anti-sense RNA on neurula injected at the 2 cell stage in one blastomere with either GFP (1 and 4), eif6 (2 and 5) or S235A (3 and 6). In GFP injected embryos (1 and 4), the hybridization intensity and pattern is identical in the embryo dorsal portion (1) and in the eye field territory (4, arrows) of both sides. In both eif6 (2 and 5) and S235A (3−6) injected samples, gipc2 is expressed at a lower level in the injected side than in the un-injected side, in particular in the eye field territory (arrows). βgal mRNA was visualized with Red-Gal. b. Real-time PCR using embryos injected with increasing concentration of eif6 (100, 200 and 400 pg) shows a highly significant (P<0.001) dose-dependent decrease of gipc2 mRNA. c. Real-time PCR in eif6 morphants showing a highly significant increase of gipc2 mRNA (P<0001) in contrast to the control mis-paired morpholino.
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