| |
Fig. 2. Expression pattern of NKL in mouse, chick and Xenopus
embryos. (A-C) In mouse, mNKL is expressed in the intermediate
zone (arrows) of E10.5 hindbrain (A) and spinal cord (B), as well
as in the DRG (arrowhead in B). At E12.5 mNKL is expressed in a
narrow band of cells in the ventricular zone (vz) (C). In chick stage
25 embryos (D) cNKL is expressed in the intermediate zone
(arrow) of the spinal cord as it is in the E10.5 mouse. (E) In
Xenopus stage 13.5 embryos staining is apparent in the neural plate
in lateral (L) and medial (M) stripes of primary neurons. (F) An
anterior view of a stage 19 embryo indicates NKL hybridization in
the trigeminal ganglion (arrow). (G) A dorsal view of columns of
NKL-positive cells in the neuraxis (arrow). Note also the onset of
NKL staining in the branchial arches (arrowhead). In tailbud stage
(32) embryos NKL staining is seen in the neuraxis, the head, the
eye and the branchial arches (H). (I,J) Transverse vibratome
sections taken immediately posterior to the hindbrain through the
neural tube of stage 24 embryos stained for NKL (I) and N-tubulin
(J). |
