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Figure 4. Expression of Gremlin in regenerating WT and N1 limbs and during development Expression of Gremlin in regenerating WT and N1 limbs and during development. Gene expression in regenerating wild type (WT) and N1 limbs and embryo tissue. (A-J) In situ hybridisation showing Gremlin expression in the regeneration bud. (L-O) Unoperated limb buds illustrating Gremlin expression during limb development. (K) In situ hybridisation showing Gremlin expression in a stage 32 embryo, the expression pattern is consistent with previously published Gremlin embryo expression [30]. White arrowheads indicate approximate amputation plane, scale bar in A applies to panels A-J and scale bar in O to pan- els L-O. In limb panels (A-J, L-O) posterior is uppermost, and distal to the left, dr = days of regeneration. In K, anterior is to the left and dorsal uppermost. White asterisks mark areas of Gremlin expression that are developmental and unrelated to regeneration. |
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grem1 (gremlin 1) gene expression in Xenopus laevis embryo, via in situ hybridization, NF stage 32 embryo, lateral view, anterior left, dorsal up. |
