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Fig. 1. Analysis of XGRIP2.1 expression by whole mount in situ hybridization and RT-PCR. (A–F) The localization of XGRIP2.1 mRNA in Xenopus oocytes and embryos was analyzed by whole mount in situ hybridization, followed by vibratome sectioning (A′–D′). In stage I oocytes, XGRIP2.1 mRNA was detected in the mitochondrial cloud (A, A′) and at early stage III at the tip of the vegetal cortex (B, B′). At the 2-cell stage of embryogenesis, XGRIP2.1 mRNA is enriched in granular patches of germ plasm at the vegetal pole (C, C′, vegetal pole view). At gastrula stage, the transcript was detected in isolated cells within the endoderm (D, blastoporus view, arrow in panel D′). At tailbud stage, XGRIP2.1 is specific to the migrating germ cells (E, lateral view of the embryo with the head to the left). XGRIP2.1 and Xpat RNAs colocalize in migrating PGCs at tailbud stage of embryogenesis. Embryos were sequentially stained for XGRIP2.1 (blue) and Xpat (red, magnified view in the inset, the first staining of the same embryo) (F, lateral view of the embryo with the head to the left). RT-PCR analysis of the temporal expression during embryogenesis (G) and tissue-specific expression of XGRIP2.1 (H). Total RNA for RT-PCR was prepared from embryos or from adult tissues. Abbreviations: Gv—germinal vesicle, Mc—mitochondrial cloud, Vc—vegetal cortex, Bc—blastocoel, B—brain, O—ovary, E—eye, Sk—skin, M—muscle, F—fat, K—kidney, G—gall bladder, Sp—spleen, I—intestine, T—testes, Li—liver, Sm—stomach, Lu—lung, P—pancreas, H—heart, Sc—spinal cord. |
