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FIG. 3. Whole mount in situ hybridization pattern of Xbap [cc: now called nkx3-1] (A, B, C, D, E, and F) and gsc (G, H, I, J, and K) and Alcian blue staining to visualize cartilage (L). All embryos were photographed at 701 magnification except for (D) at 601. (A) Lateral view of a stage 36 embryo showing both regions of facial staining and the region of gut expression (indicated by g in the figure). (B) Anterior view of the embryo pictured in (A). The paired anterior regions of expression lie on either side of the future mouth opening (M). (C) Dorsal view of a stage 37 embryo showing the forked appearance of the Xbap staining. (D) Lateral view of the left side of a stage 42 embryo showing staining of the anterior gut tube and facial staining. (E) Dorsal view of a stage 40 embryo. Xbap staining now marks crescent-shaped regions on either side of the future mouth opening (M). (F) Dorsal view of the embryo shown in (D). The region of staining corresponding to the palatoquadrate cartilage (PQ) is indicated. (G) Lateral view of a stage 36 embryo showing gsc expression in the face. (H) Anterior view of the embryo pictured in (G). Compare the gsc staining pattern with that of Xbap in (B) and note the vertical nature of the bilateral patches of staining and the broad domain of expression of the midline staining. (I) Dorsal view of the embryo shown in G and H. (J) Lateral view of a stage 42 embryo stained for gsc transcript. (K) Dorsal view of the embryo pictured in J showing the convergence of the gsc staining at the embryonic midline. (L) Ventral view of the head of a stage 43 embryo stained for cartilage. Compare the location of the palatoquadrate cartilage (PQ) with that of Xbap staining in similar stage embryos (E and F). Meckel cartilage (MC), ceratohyal cartilage (CH), and basihyobranchial cartilage (BHB) are also shown. |
