| |
Figure 7. Immunofluorescence microscopy showing the distribution
of the actin-modulating protein of Mr '~ 93,000 in cryostat sections
of Xenopus laevis oocytes (a and b) and intestine (c). The sections were fixed with 10% formaldehyde and treated with antibodies
(antiserum diluted 1:10) against the actin-modulating protein (a).
General staining of the oocyte cortex (demarcated by brackets) and
ooplasm (b, phase-contrast image coresponding to a). O, ooplasm.
In the intestine (c) epithelial cells (E) and smooth muscle (M) were
stained intensely, whereas cells of the lamina propria (LP) were
weakly, if at all, reactive. Bars, 50 Ixm. |
